limpca 1.0.0
This package was created to analyse models with high-dimensional data and a multi-factor design of experiment. limpca
stands for linear modeling of high-dimensional designed data based on the ASCA (ANOVA-Simultaneous Component Analysis) and APCA (ANOVA-Principal Component Analysis) family of methods. These methods combine ANOVA with a General Linear Model (GLM) decomposition and PCA. They provide powerful visualization tools for multivariate structures in the space of each effect of the statistical model linked to the experimental design. Details on the methods used and the package implementation can be found in the articles of Thiel, Féraud, and Govaerts (2017), Guisset, Martin, and Govaerts (2019) and Thiel et al. (2023).
Therefore, ASCA/APCA are highly informative modeling and visualisation tools to analyse -omics data tables in a multivariate framework and act as a complement to differential expression analyses methods such as limma
(Ritchie et al. (2015)).
Get started with limpca
(this vignette): This vignette is a short application of limpca
on the UCH
dataset with data visualisation, exploration (PCA), GLM decomposition and ASCA modelling. The ASCA model used in this example is a three-way ANOVA with fixed effects.
Analysis of the UCH dataset with limpca: This vignette is an extensive application of limpca
on the UCH
dataset with data visualisation, exploration (PCA), GLM decomposition and ASCA/APCA/ASCA-E modelling. The applied model is a three-way ANOVA with fixed effects. This document presents all the usual steps of the analysis, from importing the data to visualising the results.
Analysis of the Trout dataset with limpca: This vignette is an extensive application of limpca
on the Trout
dataset with data visualisation, exploration (PCA), GLM decomposition and ASCA/APCA/ASCA-E modelling. The applied model involves three main effects and their two-way interaction terms. It also compares the results of ASCA to a univariate ANOVA modeling.
limpca
packagelimpca
can be installed from Bioconductor:
if (!requireNamespace("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("limpca")
And then loaded into your R session:
library("limpca")
For any enquiry, you can send an email to the package authors: [email protected] ; [email protected] or [email protected]
UCH
datasetIn order to use the limpca core functions, the data need to be formatted as a list (informally called an lmpDataList) with the following elements: outcomes
(multivariate matrix), design
(data.frame) and formula
(character string).
The UCH
data set is already formatted appropriately and can be loaded from limpca
with the data
function.
data("UCH")
str(UCH)
#> List of 3
#> $ design :'data.frame': 34 obs. of 5 variables:
#> ..$ Hippurate: Factor w/ 3 levels "0","1","2": 1 1 1 1 1 1 2 2 2 2 ...
#> ..$ Citrate : Factor w/ 3 levels "0","2","4": 1 1 2 2 3 3 1 1 2 2 ...
#> ..$ Dilution : Factor w/ 1 level "diluted": 1 1 1 1 1 1 1 1 1 1 ...
#> ..$ Day : Factor w/ 2 levels "2","3": 1 1 1 1 1 1 1 1 1 1 ...
#> ..$ Time : Factor w/ 2 levels "1","2": 1 2 1 2 1 2 1 2 1 2 ...
#> $ outcomes: num [1:34, 1:600] 0.0312 0.0581 0.027 0.0341 0.0406 ...
#> ..- attr(*, "dimnames")=List of 2
#> .. ..$ : chr [1:34] "M2C00D2R1" "M2C00D2R2" "M2C02D2R1" "M2C02D2R2" ...
#> .. ..$ X1: chr [1:600] "9.9917004" "9.9753204" "9.9590624" "9.9427436" ...
#> $ formula : chr "outcomes ~ Hippurate + Citrate + Time + Hippurate:Citrate + Time:Hippurate + Time:Citrate + Hippurate:Citrate:Time"
Alternatively, the lmpDataList can be created with the function data2LmpDataList
:
UCH2 <- data2LmpDataList(
outcomes = UCH$outcomes,
design = UCH$design,
formula = UCH$formula
)
#> | dim outcomes: 34x600
#> | formula: ~ Hippurate + Citrate + Time + Hippurate:Citrate + Time:Hippurate + Time:Citrate + Hippurate:Citrate:Time
#> | design variables (5):
#> * Hippurate (factor)
#> * Citrate (factor)
#> * Dilution (factor)
#> * Day (factor)
#> * Time (factor)
SummarizedExperiment
:se <- SummarizedExperiment(
assays = list(
counts = t(UCH$outcomes)), colData = UCH$design,
metadata = list(formula = UCH$formula)
)
UCH3 <- data2LmpDataList(se, assay_name = "counts")
#> | dim outcomes: 34x600
#> | formula: ~ Hippurate + Citrate + Time + Hippurate:Citrate + Time:Hippurate + Time:Citrate + Hippurate:Citrate:Time
#> | design variables (5):
#> * Hippurate (factor)
#> * Citrate (factor)
#> * Dilution (factor)
#> * Day (factor)
#> * Time (factor)
SummarizedExperiment
is a generic data container that stores rectangular matrices of experimental results. See Morgan et al. (2023) for more information.
The design can be visualised with plotDesign()
.
# design
plotDesign(
design = UCH$design, x = "Hippurate",
y = "Citrate", rows = "Time",
title = "Design of the UCH dataset"
)
# row 3 of outcomes
plotLine(
Y = UCH$outcomes,
title = "H-NMR spectrum",
rows = c(3),
xlab = "ppm",
ylab = "Intensity"
)
ResPCA <- pcaBySvd(UCH$outcomes)
pcaScreePlot(ResPCA, nPC = 6)
pcaScorePlot(
resPcaBySvd = ResPCA, axes = c(1, 2),
title = "PCA scores plot: PC1 and PC2",
design = UCH$design,
color = "Hippurate", shape = "Citrate",
points_labs_rn = FALSE
)
# Model matrix generation
resMM <- lmpModelMatrix(UCH)
# Model estimation and effect matrices decomposition
resEM <- lmpEffectMatrices(resMM)
# Effects importance
resEM$varPercentagesPlot
# Bootstrap tests
resBT <- lmpBootstrapTests(resLmpEffectMatrices = resEM, nboot = 100)
resBT$resultsTable
#> % of variance (T III) Bootstrap p-values
#> Hippurate 39.31 < 0.01
#> Citrate 29.91 < 0.01
#> Time 16.24 < 0.01
#> Hippurate:Citrate 1.54 0.12
#> Hippurate:Time 6.23 < 0.01
#> Citrate:Time 0.54 0.39
#> Hippurate:Citrate:Time 1.68 0.08
#> Residuals 4.30 -
# ASCA decomposition
resASCA <- lmpPcaEffects(resLmpEffectMatrices = resEM, method = "ASCA")
# Scores Plot for the hippurate
lmpScorePlot(resASCA,
effectNames = "Hippurate",
color = "Hippurate", shape = "Hippurate"
)
# Loadings Plot for the hippurate
lmpLoading1dPlot(resASCA,
effectNames = c("Hippurate"),
axes = 1, xlab = "ppm"
)
# Scores ScatterPlot matrix
lmpScoreScatterPlotM(resASCA,
PCdim = c(1, 1, 1, 1, 1, 1, 1, 2),
modelAbbrev = TRUE,
varname.colorup = "Citrate",
varname.colordown = "Time",
varname.pchup = "Hippurate",
varname.pchdown = "Time",
title = "ASCA scores scatterplot matrix"
)
sessionInfo()
#> R version 4.4.0 beta (2024-04-15 r86425)
#> Platform: x86_64-pc-linux-gnu
#> Running under: Ubuntu 22.04.4 LTS
#>
#> Matrix products: default
#> BLAS: /home/biocbuild/bbs-3.19-bioc/R/lib/libRblas.so
#> LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.10.0
#>
#> locale:
#> [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
#> [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
#> [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
#> [7] LC_PAPER=en_US.UTF-8 LC_NAME=C
#> [9] LC_ADDRESS=C LC_TELEPHONE=C
#> [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
#>
#> time zone: America/New_York
#> tzcode source: system (glibc)
#>
#> attached base packages:
#> [1] stats4 stats graphics grDevices utils datasets methods
#> [8] base
#>
#> other attached packages:
#> [1] SummarizedExperiment_1.34.0 Biobase_2.64.0
#> [3] GenomicRanges_1.56.0 GenomeInfoDb_1.40.0
#> [5] IRanges_2.38.0 S4Vectors_0.42.0
#> [7] BiocGenerics_0.50.0 MatrixGenerics_1.16.0
#> [9] matrixStats_1.3.0 limpca_1.0.0
#> [11] BiocStyle_2.32.0
#>
#> loaded via a namespace (and not attached):
#> [1] gtable_0.3.5 xfun_0.43 bslib_0.7.0
#> [4] ggplot2_3.5.1 ggrepel_0.9.5 lattice_0.22-6
#> [7] vctrs_0.6.5 tools_4.4.0 generics_0.1.3
#> [10] parallel_4.4.0 tibble_3.2.1 fansi_1.0.6
#> [13] highr_0.10 pkgconfig_2.0.3 Matrix_1.7-0
#> [16] tidyverse_2.0.0 lifecycle_1.0.4 GenomeInfoDbData_1.2.12
#> [19] farver_2.1.1 compiler_4.4.0 stringr_1.5.1
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#> [25] codetools_0.2-20 htmltools_0.5.8.1 sass_0.4.9
#> [28] yaml_2.3.8 tidyr_1.3.1 pillar_1.9.0
#> [31] crayon_1.5.2 jquerylib_0.1.4 DelayedArray_0.30.0
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#> [37] abind_1.4-5 foreach_1.5.2 tidyselect_1.2.1
#> [40] digest_0.6.35 stringi_1.8.3 purrr_1.0.2
#> [43] dplyr_1.1.4 reshape2_1.4.4 bookdown_0.39
#> [46] labeling_0.4.3 fastmap_1.1.1 grid_4.4.0
#> [49] colorspace_2.1-0 cli_3.6.2 SparseArray_1.4.0
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#> [58] rmarkdown_2.26 XVector_0.44.0 httr_1.4.7
#> [61] evaluate_0.23 knitr_1.46 doParallel_1.0.17
#> [64] rlang_1.1.3 Rcpp_1.0.12 glue_1.7.0
#> [67] BiocManager_1.30.22 jsonlite_1.8.8 R6_2.5.1
#> [70] plyr_1.8.9 zlibbioc_1.50.0
Guisset, Séverine, Manon Martin, and Bernadette Govaerts. 2019. “Comparison of Parafasca, Acomdim, and Amopls Approaches in the Multivariate Glm Modelling of Multi-Factorial Designs.” Chemometrics and Intelligent Laboratory Systems 184: 44–63. https://doi.org/https://doi.org/10.1016/j.chemolab.2018.11.006.
Morgan, Martin, Valerie Obenchain, Jim Hester, and Hervé Pagès. 2023. SummarizedExperiment: SummarizedExperiment Container. https://doi.org/10.18129/B9.bioc.SummarizedExperiment.
Ritchie, Matthew E, Belinda Phipson, Di Wu, Yifang Hu, Charity W Law, Wei Shi, and Gordon K Smyth. 2015. “limma Powers Differential Expression Analyses for RNA-Sequencing and Microarray Studies.” Nucleic Acids Research 43 (7): e47. https://doi.org/10.1093/nar/gkv007.
Thiel, Michel, Nadia Benaiche, Manon Martin, Sébastien Franceschini, Robin Van Oirbeek, and Bernadette Govaerts. 2023. “Limpca: An R Package for the Linear Modeling of High-Dimensional Designed Data Based on Asca/Apca Family of Methods.” Journal of Chemometrics 37 (7): e3482. https://doi.org/https://doi.org/10.1002/cem.3482.
Thiel, Michel, Baptiste Féraud, and Bernadette Govaerts. 2017. “ASCA+ and Apca+: Extensions of Asca and Apca in the Analysis of Unbalanced Multifactorial Designs.” Journal of Chemometrics 31 (6): e2895. https://doi.org/https://doi.org/10.1002/cem.2895.